Our pipelines can be used individually or in tandem with each other.
Each pipeline is fully automated, optimized, and validated specifically for quantifying the topology and dynamics of mitochondrial networks in budding yeast. Each pipeline has also been tested across a range of imaging platforms (Zeiss Spinning disk, GE SIM OMX, LSM780, LSM770, LSM880) and fluorophores used to label mitochondria (BFP, EGFP, mCherry, mScarlet).
Rapid automated identification, classification, and cartography of yeast cells…
Identifies organelle regional localization and migration within the yeast cell…
Fast ML driven 3D volumetric segmentation of fluorescently labeled mitochondria…
Runs common network analysis algorithms and structure-to-structure analysis on…
Analyze mitochondrial network dynamics over time (velocity, MSD) and structural…
Quantification of mitochondrial content (i.e. membrane potential dyes,…